Quantitation of phosphatidylserine-exposing platelets in platelet concentrate prepared in routine blood transfusion laboratory

Quantitative determination of the binding of beta2-glycoprotein I and prothrombin to phosphatidylserine-exposing blood platelets.

The plasma protein beta2GPI (beta2-glycoprotein I) has been proposed to mediate phagocytosis of apoptotic cells and to play a role in the antiphospholipid syndrome. This suggestion is based mainly on the presumption that beta2GPI has an appreciable interaction with PS (phosphatidylserine)-exposing cell membranes. However, quantitative data on the binding of beta2GPI to PS-exposing cells under p...

A Novel Platelet Concentrate: Titanium-Prepared Platelet-Rich Fibrin

We developed a new product called titanium-prepared platelet-rich fibrin (T-PRF). The T-PRF method is based on the hypothesis that titanium may be more effective in activating platelets than the silica activators used with glass tubes in Chouckroun's leukocyte- and platelet-rich fibrin (L-PRF) method. In this study, we aimed to define the structural characteristics of T-PRF and compare it with ...

Laboratory evaluation of platelets for transfusion.

تاثیر فیلتراسیون پیش از ذخیره کردن بر فعال شدن پلاکت‌ها در فراورده‌های پلاکتی

 Preparation conditions and platelet storage for transfusion may cause platelet activation, which contributes to decreased ability of stored platelet to function and survive in vivo after transfusion compared with that seen with freshly prepared platelets. Using flowcytometry, we investigated platelet membrane expression of CD62P, CD63 in platelet stored for up to 3 days under standard blood ba...

Phosphatidylserine exposing-platelets and microparticles promote procoagulant activity in colon cancer patients.

BACKGROUND Colon cancer is invariably accompanied by altered coagulation activity; however, the precise role of phosphatidylserine (PS) in the hypercoagulable state of colon cancer patients remains unclear. We explored the exposure of PS on platelets and microparticles (MPs), and evaluate its role in procoagulant activity in colon cancer patients. METHODS PS-positive platelets and MPs, mainly...